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Updated: Mar 25, 2026
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Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
Published on: May 23, 2025
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Mitochondrial damage-associated molecular patterns as potential proinflammatory mediators in post-platelet
Kazuta Yasui1, Nobuki Matsuyama1, Ayumu Kuroishi1
1Japanese Red Cross Kinki Block Blood Center, Ibaraki-City, Osaka, Japan.
Transfusion
|February 28, 2016
Summary
Mitochondrial damage-associated molecular patterns (DAMPs) from platelets may cause nonhemolytic transfusion reactions (NHTRs). Higher levels of these platelet-derived DAMPs were found in reactions, suggesting a key role in inflammation.
Area of Science:
- Immunology
- Hematology
- Transfusion Medicine
Background:
- Nonhemolytic transfusion reactions (NHTRs), common with platelet concentrates (PCs), lack understood mechanisms.
- Mitochondria-derived damage-associated molecular patterns (DAMPs) are implicated in innate immune cell inflammation.
- Platelets (PLTs) are a significant source of circulating mitochondria.
Purpose of the Study:
- To investigate the potential role of mitochondrial DAMPs in the pathogenesis of NHTRs.
- To quantify mitochondrial DAMPs in blood components associated with NHTRs.
Main Methods:
- Mitochondrial DNA (mtDNA) copy number quantified via real-time PCR as an index of mitochondrial DAMPs.
- In vitro whole blood cell culture assays assessed activation of neutrophils, monocytes, and basophils by mitochondrial DAMPs.
Main Results:
- Mean total mtDNA concentration was highest in PCs, followed by fresh-frozen plasma and red blood cells.
- NHTR-associated PCs exhibited significantly higher mtDNA levels compared to control PCs.
- Mitochondrial DAMPs in NHTR PCs were sufficient to activate neutrophils, monocytes, and basophils.
Conclusions:
- Platelet-derived mitochondrial DAMPs are identified as potential risk factors for NHTRs.
- This study highlights the contribution of mitochondrial components to transfusion-related immune responses.
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