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Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

840
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
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NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

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When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.7K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.7K
Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

1.6K
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
1.6K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

2.1K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
2.1K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.5K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

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Optimizing dissolution dynamic nuclear polarization.

Aurélien Bornet1, Sami Jannin2

  • 1Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), Batochime, CH-1015 Lausanne, Switzerland.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|February 28, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a new dissolution dynamic nuclear polarization (d-DNP) method using specific polarizing agents and advanced techniques. This approach enhances nuclear spin polarization for improved sensitivity in various applications.

Keywords:
Cross polarizationDissolution dynamic nuclear polarizationHyperpolarization

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Area of Science:

  • Nuclear Magnetic Resonance Spectroscopy
  • Physical Chemistry
  • Biophysics

Background:

  • Dynamic Nuclear Polarization (DNP) enhances NMR signal sensitivity.
  • Conventional DNP methods have limitations in efficiency and application scope.
  • Advancements in DNP are crucial for pushing the boundaries of molecular detection.

Purpose of the Study:

  • To review recent developments in dissolution dynamic nuclear polarization (d-DNP).
  • To present a modified d-DNP protocol that deviates from conventional techniques.
  • To highlight the key components of the improved d-DNP approach.

Main Methods:

  • Utilizing broad line polarizing agents for efficient 1H spin polarization.
  • Employing high magnetic fields (6.7 T and above).
  • Implementing microwave frequency modulation and 1H-13C cross-polarization.
  • Transferring hyperpolarized solutions via a magnetic tunnel.

Main Results:

  • Demonstration of an efficient d-DNP protocol.
  • Successful polarization of 1H spins using broad line agents.
  • Effective transfer of hyperpolarized solutions.

Conclusions:

  • The modified d-DNP approach offers significant improvements over conventional methods.
  • This technique expands the utility of d-DNP in scientific research.
  • Further development in d-DNP promises enhanced sensitivity for molecular studies.