Related Experiment Video
Updated: Mar 25, 2026
![Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F54014.jpg&w=3840&q=50)
Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
Published on: May 19, 2016
S-nitrosylation regulates VE-cadherin phosphorylation and internalization in microvascular permeability
Anita Guequén1, Rodrigo Carrasco1, Patricia Zamorano1
1Instituto de Inmunología, Universidad Austral de Chile, Valdivia, Chile;
Abstract:
The adherens junction complex, composed mainly of vascular endothelial (VE)-cadherin, β-catenin, p120, and γ-catenin, is the main element of the endothelial barrier in postcapillary venules.S-nitrosylation of β-catenin and p120 is an important step in proinflammatory agents-induced hyperpermeability. We investigated in vitro and in vivo whether or not VE-cadherin isS-nitrosylated using platelet-activating factor (PAF) as agonist. We report that PAF-stimulates S-nitrosylation of VE-cadherin, which disrupts its association with β-catenin. In addition, based on inhibition of nitric oxide production, our results strongly suggest that S-nitrosylation is required for VE-cadherin phosphorylation on tyrosine and for its internalization. Our results unveil an important mechanism to regulate phosphorylation of junctional proteins in association with S-nitrosylation.
More Related Videos
Related Concept Videos
Regulation of Angiogenesis and Blood Supply
Cadherins in Tissue Organization
Cell Sorting During Development
Cell sorting plays an...
Intracellular Signaling Affects Focal Adhesions
Some...
Adherens Junctions
Adherens Junctions are Dynamic
Nitric Oxide Signaling Pathway
Regulation of Nuclear Protein Sorting

