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Activation of TIM1 induces colon cancer cell apoptosis via modulating Fas ligand expression

Hao Wang1, Xueyan Zhang1, Wenjing Sun2

  • 1Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150086, China.

Biochemical and Biophysical Research Communications
|February 28, 2016
PubMed

Insights

Activation of TIM1 in colon cancer cells induces apoptosis, suggesting TIM1 as a potential therapeutic target for colon cancer treatment. This study explored TIM1

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • The precise mechanisms driving colon cancer development remain incompletely understood.
  • Tumor-induced myeloidexpressed gene 1 (TIM1) has been implicated in various human cancers.
  • Investigating TIM1's role offers potential for novel therapeutic strategies in colon cancer.

Purpose of the Study:

  • To elucidate the function of TIM1 activation in suppressing human colon cancer cell proliferation.
  • To determine if TIM1 can serve as a therapeutic target for colon cancer.

Main Methods:

  • Cultured human colon cancer cell lines (HT29 and T84).
  • Assessed TIM1 expression using real-time RT-PCR and Western blotting.
  • Activated TIM1 with recombinant TIM4, analyzed apoptosis via flow cytometry, and examined FasL promoter activity using chromatin immunoprecipitation.

Main Results:

  • Both HT29 and T84 colon cancer cells express TIM1.
  • TIM1 activation by TIM4 significantly enhanced colon cancer cell apoptosis.
  • TIM4 treatment increased FasL expression, and blocking Fas/FasL pathways abrogated TIM4-induced apoptosis.

Conclusions:

  • TIM1 is expressed in human colon cancer cell lines HT29 and T84.
  • TIM1 activation effectively induces apoptosis in colon cancer cells.
  • TIM1 represents a promising novel therapeutic target for colon cancer intervention.
Keywords:
CancerColonFasLHT29 cellT84 cellTIM1TIM4

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