A highly sensitive and selective impedimetric aptasensor for interleukin-17 receptor A

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Analytical Chemistry

Background:

• Interleukin-17 receptor A (IL-17RA) is a key biomarker in various diseases, particularly autoimmune conditions.
• Accurate and sensitive detection of IL-17RA is crucial for disease diagnosis and management.
• Existing detection methods, such as ELISA, have limitations in sensitivity and efficiency.

Purpose of the Study:

• To develop a highly sensitive and selective electrochemical biosensor for Interleukin-17 receptor A (IL-17RA) detection.
• To utilize an aptamer-based approach for quantitative IL-17RA measurement.
• To establish a novel diagnostic platform for IL-17RA-mediated diseases.

Main Methods:

• Fabrication of an electrochemical biosensor via electrodeposition of gold nanoparticles.
• Immobilization of an IL-17RA aptamer for specific target recognition.
• Quantitative detection of IL-17RA using electrochemical impedance spectroscopy (EIS).

Main Results:

• The aptasensor demonstrated high sensitivity and selectivity for IL-17RA detection.
• Achieved a wide dynamic range (10–10,000 pg/mL) and a low detection limit (2.13 pg/mL).
• Successfully detected IL-17RA in human serum and differentiated HL-60 cells, showing clinical applicability in asthma patient neutrophils.

Conclusions:

• The developed aptasensor provides a superior analytical performance compared to conventional ELISA kits.
• This novel biosensor represents a promising diagnostic platform for IL-17RA-associated diseases.
• The aptasensor's ability to detect IL-17RA in complex biological samples highlights its clinical potential.