Related Experiment Video
Updated: Mar 25, 2026

Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Published on: May 26, 2023
Calcium signaling in human pluripotent stem cells
Ágota Apáti1, Tünde Berecz1, Balázs Sarkadi2
1Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
Calcium signaling is crucial for human pluripotent stem cells (hPSCs) but remains understudied. This review covers methods and pathways for studying calcium responses in hPSCs, including naïve and fuzzy states.
Area of Science:
- Stem cell biology
- Cellular physiology
Background:
- Human pluripotent stem cells (hPSCs) are vital for research and regenerative medicine.
- Calcium homeostasis and signaling regulate critical cellular processes like proliferation, differentiation, and apoptosis.
- Detailed characterization of calcium dynamics in hPSCs is lacking.
Purpose of the Study:
- To review methods for studying calcium responses in hPSCs.
- To discuss calcium signaling pathways in hPSCs and compare them to mouse counterparts.
- To explore the role of calcium homeostasis in novel hPSC states (naïve and fuzzy).
Main Methods:
- Focus on fluorescent calcium indicator proteins and imaging techniques.
- Application of these methods in pluripotent human stem cells.
- Comparative analysis of calcium pathways between human and mouse pluripotent stem cells.
Main Results:
- Methods for assessing short- and long-term calcium responses in hPSCs are available.
- Potential regulatory pathways for calcium signaling in hPSCs are identified.
- Calcium homeostasis may play a role in naïve and fuzzy hPSC states.
Conclusions:
- Understanding calcium signaling in hPSCs is essential for their application in various fields.
- Further research is needed to elucidate calcium's role in different hPSC states.
- Comparative studies with mouse models provide valuable insights.
More Related Videos
10:01High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening
Published on: March 31, 2022
07:33A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
Published on: August 3, 2015
Related Concept Videos
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Somatic...
Chromatin Modification in iPS Cells
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Maintenance of the ES Cell State