Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Structure01:23

RNA Structure

29.7K
29.7K
RNA Structure01:23

RNA Structure

80.3K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
80.3K
RNA Structure01:19

RNA Structure

8.2K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.2K
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
Nucleic Acid Structure01:25

Nucleic Acid Structure

10.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
10.1K
Leaky Scanning02:28

Leaky Scanning

5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

XIST RNA-protein complex in female-biased autoimmunity: From molecular scaffolds to new clinical biomarkers.

The Journal of investigative dermatology·2026
Same author

Somatic mutations reveal the ontogeny of human microglia.

bioRxiv : the preprint server for biology·2026
Same author

Correction: Annotation of nuclear lncRNAs based on chromatin interactions.

PloS one·2026
Same author

Fast and accurate resolution of ecDNA sequence using Cycle-Extractor.

bioRxiv : the preprint server for biology·2026
Same author

A recipe for chaos: Extrachromosomal DNA and the hallmarks of cancer.

Cell·2026
Same author

Accurate prediction of ecDNA in interphase cancer cells using deep neural networks.

Communications biology·2026
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

Related Experiment Video

Updated: Mar 25, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.4K

Decoding the RNA structurome.

Zhipeng Lu1, Howard Y Chang1

  • 1Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, CA 94305, United States.

Current Opinion in Structural Biology
|March 1, 2016
PubMed
Summary
This summary is machine-generated.

Understanding RNA structure is key to its function. New high-throughput sequencing methods allow us to map the RNA structurome, revealing crucial insights into RNA

More Related Videos

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.7K
RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

32.5K

Related Experiment Videos

Last Updated: Mar 25, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.4K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.7K
RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

32.5K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • RNA molecules perform critical architectural, regulatory, and catalytic roles.
  • The three-dimensional structure of RNA dictates its biological functions.
  • Understanding RNA structure is fundamental to deciphering gene expression and regulation.

Purpose of the Study:

  • To review current state-of-the-art technologies for probing the RNA structurome.
  • To highlight key insights gained from RNA structurome studies.
  • To identify limitations of existing methods and propose future research directions.

Main Methods:

  • High-throughput sequencing technologies for RNA structure probing.
  • Development of transcriptome-wide RNA structure mapping techniques.
  • Analysis of large-scale RNA structurome datasets.

Main Results:

  • Recent advances enable comprehensive mapping of RNA structures across the transcriptome (RNA structurome).
  • These methods provide unprecedented insights into RNA folding and function in vivo.
  • The review consolidates current technological capabilities and findings in the field.

Conclusions:

  • The RNA structurome is a critical layer of gene regulation.
  • Advancements in sequencing technologies have revolutionized RNA structure analysis.
  • Future improvements in methods are needed to overcome current limitations and expand our understanding.