Direct visualization of replication dynamics in early zebrafish embryos
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Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
Published on: July 15, 2016
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Kenji Kuriya1, Eriko Higashiyama1, Eriko Avşar-Ban2
1a Laboratory of Molecular & Cellular Biology, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
Bioscience, Biotechnology, and Biochemistry
|March 1, 2016
Abstract:
We analyzed DNA replication in early zebrafish embryos. The replicating DNA of whole embryos was labeled with the thymidine analog 5-ethynyl-2'-deoxyuridine (EdU), and spatial regulation of replication sites was visualized in single embryo-derived cells. The results unveiled uncharacterized replication dynamics during zebrafish early embryogenesis.
