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Updated: Mar 25, 2026
Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
Published on: December 23, 2022
Single step purification of concanavalin A (Con A) and bio-sugar production from jack bean using glucosylated
Ho Myeong Kim1, Eun Jin Cho2, Hyeun-Jong Bae3
1Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Abstract:
Jack bean (JB, Canavalia ensiformis) is the source of bio-based products, such as proteins and bio-sugars that contribute to modern molecular biology and biomedical research. In this study, the use of jack bean was evaluated as a source for concanavalin A (Con A) and bio-sugar production. A novel method for purifying Con A from JBs was successfully developed using a glucosylated magnetic nano matrix (GMNM) as a physical support, which facilitated easy separation and purification of Con A. In addition, the enzymatic conversion rate of 2% (w/v) Con A extracted residue to bio-sugar was 98.4%. Therefore, this new approach for the production of Con A and bio-sugar is potentially useful for obtaining bio-based products from jack bean.
