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A chirality change in XPC- and Sfi1-derived peptides affects their affinity for centrin
Dora Grecu1, Victor Paul Raj Irudayaraj2, Juan Martinez-Sanz3
1Institut Curie-Centre de Recherche, F-91405 Orsay Cédex, France; Institut National de la Santé et de la Recherche Médicale (INSERM) U759, F-91405 Orsay Cédex, France.
Peptides
|March 1, 2016
Summary
This study shows that D-peptides bind centrin with lower affinity than L-peptides, with binding strength varying by sequence orientation. This suggests D-peptides may inhibit centrin binding to cellular targets.
Area of Science:
- Biochemistry
- Molecular Biology
- Protein-protein interactions
Background:
- Centrin is a Ca(2+)-binding protein that interacts with hydrophobic motifs in cellular targets like XPC, Sfi1, and Sac3.
- Centrin binding orientation differs between targets (XPC vs. Sfi1/Sac3).
- D-peptides are being explored for therapeutic applications.
Purpose of the Study:
- To investigate the centrin-binding properties of D-peptides.
- To compare the binding affinities of D-peptides versus L-peptides to centrin.
- To assess the potential of D-peptides for inhibiting centrin-target interactions.
Main Methods:
- Isothermal titration calorimetry was used to measure peptide-centrin binding affinities.
- Analysis of centrin binding to peptides derived from XPC, Sfi1, and Sac3.
- Comparison of binding affinities for both L- and D-enantiomers of peptides.
Main Results:
- Chirality significantly impacts centrin-binding ability, dependent on peptide sequence orientation.
- D-XPC-P10 binds Ca(2+)-dependently but with a ~2000-fold lower affinity than L-XPC-P10.
- D-peptides generally exhibit reduced affinity for centrin compared to L-peptides.
Conclusions:
- The affinity of D-peptides for centrin is sequence-orientation dependent.
- The reduced affinity of D-peptides suggests their potential as inhibitors of centrin-target interactions.
- D-peptides represent a promising therapeutic strategy for modulating centrin function.
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