Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammatory Response01:28

Inflammatory Response

18.4K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
18.4K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.3K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.3K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

13.8K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.8K
T Cell Types and Functions01:24

T Cell Types and Functions

3.2K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.2K
General Transcription Factors01:30

General Transcription Factors

7.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.5K
Inflammation01:38

Inflammation

64.0K
Overview
64.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of the actin remodeling protein Flightless-1 impairs CD8 and regulatory T cell function.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Neutrophil Transcriptomic Changes in Severe Sterile Vasoplegic Syndrome Resemble a Distinct Molecular Subtype of Septic Shock.

Shock (Augusta, Ga.)·2026
Same author

Adipocyte NADH dehydrogenase reverses circadian and diet-induced metabolic syndrome.

Nature metabolism·2026
Same author

Prevention of Cholestatic Liver Disease Through BCL6-FXR Enterohepatic Crosstalk.

Cellular and molecular gastroenterology and hepatology·2025
Same author

NADH dehydrogenase reverses dietary and clock metabolic syndrome.

bioRxiv : the preprint server for biology·2025
Same author

The psychophysics of remembered affordances.

Psychological research·2025

Related Experiment Video

Updated: Mar 25, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

7.0K

A Stringent Systems Approach Uncovers Gene-Specific Mechanisms Regulating Inflammation.

Ann-Jay Tong1, Xin Liu1, Brandon J Thomas1

  • 1Department of Microbiology, Immunology, and Molecular Genetics, and Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Cell
|March 1, 2016
PubMed
Summary

This study reveals unique mechanisms regulating individual genes by analyzing transcription factor binding and gene expression data. It highlights the value of detailed gene interrogation over broad systems analyses for understanding transcriptional control.

More Related Videos

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies
09:08

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Published on: June 2, 2021

2.2K
Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
06:29

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Published on: March 1, 2011

27.1K

Related Experiment Videos

Last Updated: Mar 25, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

7.0K
Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies
09:08

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Published on: June 2, 2021

2.2K
Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
06:29

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Published on: March 1, 2011

27.1K

Area of Science:

  • Molecular Biology
  • Systems Biology
  • Genomics

Background:

  • Large-scale systems analyses have advanced understanding of transcriptional networks.
  • Current methods analyzing thousands of ChIP-seq peaks or differentially expressed genes have limitations in uncovering mechanistic principles.

Purpose of the Study:

  • To uncover mechanistic principles of transcriptional control by examining quantitative signal distribution.
  • To identify relationships between transcription factor binding, motif strength, and transcription.
  • To demonstrate the value of stringent interrogation of well-defined gene sets.

Main Methods:

  • Analysis of nascent RNA sequencing (RNA-seq), ChIP-seq, and binding motif datasets.
  • Examination of quantitative signal distribution in lipid A-stimulated macrophages.
  • Focus on individual gene regulation rather than large gene clusters.

Main Results:

  • Identified unexpected relationships between in vivo transcription factor binding, motif strength, and transcription.
  • Highlighted unique regulatory mechanisms for individual genes with key biological functions.
  • Demonstrated that stringent interrogation of specific gene sets complements broader systems analyses.

Conclusions:

  • Detailed analysis of quantitative data provides mechanistic insights into transcriptional control.
  • Individual gene regulation involves unique mechanisms that are crucial for biological function.
  • A focused approach to gene set analysis is a valuable complement to large-scale systems biology studies.