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Updated: Aug 2, 2026

In vivo Measurement of Knee Extensor Muscle Function in Mice
Published on: March 4, 2021
The in vivo force-velocity relationship of the knee flexors and extensors
1Department of Surgery, College of Medicine, University of California, Irvine.
Abstract:
Previous studies have compared the torque production of the knee flexors and extensors and developed a "hamstring-quadricep" ratio. Collectively, these studies indicate that the ratio of strength between the knee flexors and extensors is a function of test speed, with the ratio increasing at the faster test speeds. Unfortunately, these studies have employed the peak torque isokinetic method for making measurements. This technique suffers from a number of problems that are biomechanical and physiological in nature. Consequently, in this study, we employed comparison of the in vivo force-velocity relationship using an angle-specific method and a gravitational correction factor to more accurately determine flexor-extensor ratios and to better describe any variations occurring as a function of test speed. Nine male subjects volunteered to participate in this study. Each of the subjects participated in two test sessions. Subjects were tested at six angular velocities of 0, 48, 96, 144, 192, 240 deg/sec. At these respective test speeds, the mean flexor torques were 95.0, 94.8, 84.1, 66.2, 56.2, and 49.2 Nm, respectively. The corresponding values for the knee extensors were 252.2, 246.5, 220.7, 184.5, 161.9, and 146.4 Nm. Normalization of these torque-velocity relationships demonstrated that the in vivo force-velocity relationships of the two muscle groups are similar at slow speeds, but become disparate at higher velocities. When examining flexor-extensor torque ratios at the various test speeds, it was found that there were significant differences (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
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