Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.5K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.5K
Autophagy01:27

Autophagy

6.1K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
6.1K
Autophagic Cell Death01:18

Autophagic Cell Death

5.0K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
5.0K
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

4.7K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
4.7K
Maturation of Endosomes01:28

Maturation of Endosomes

6.3K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
6.3K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.4K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alterations in Metabolites Associated With Umbilical Cord Blood in Monozygotic Twins Discordant for Congenital Heart Disease.

Pediatric discovery·2026
Same author

Genomic signature of repeated transitions to diurnality in spiders.

Molecular biology and evolution·2026
Same author

A negative regulator of mitochondrial complex I assembly adapts respiration to cellular energy demand.

Molecular cell·2026
Same author

Correction to: SIRT3 activation protects from nabumetone-induced mitochondrial toxicity in adult human cardiomyocytes.

Cellular and molecular life sciences : CMLS·2026
Same author

Mitochondrial proteases maintain cellular protein homeostasis and tissue integrity.

Cell & bioscience·2026
Same author

PKCα-mediated nuclear translocation of cGAS stabilizes β-catenin and drives metastasis.

Molecular cell·2026

Related Experiment Video

Updated: Mar 25, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K

Dynamin Regulates Autophagy by Modulating Lysosomal Function.

Xuefei Fang1, Jia Zhou1, Wei Liu1

  • 1Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou 310058, China.

Journal of Genetics and Genomics = Yi Chuan Xue Bao
|March 1, 2016
PubMed
Summary

Shibire (shi), a dynamin protein, is crucial for cellular homeostasis via autophagy. This study reveals shi’s essential role in lysosomal acidification, a key step in starvation-induced autophagy.

Keywords:
AutophagyDrosophilaDynaminEndocytosis

More Related Videos

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

18.8K
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

7.8K

Related Experiment Videos

Last Updated: Mar 25, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K
The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

18.8K
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

7.8K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Autophagy is a vital cellular process for homeostasis, and its malfunction is linked to human diseases.
  • Identifying novel regulators of autophagy is crucial for understanding cellular health and disease.

Purpose of the Study:

  • To identify novel genetic players involved in starvation-induced autophagy in Drosophila.
  • To elucidate the specific role of shibire (shi) in the autophagy pathway.

Main Methods:

  • Utilized a large-scale genetic screen of X-linked mutations in Drosophila fat body clones.
  • Investigated the function of shibire (shi) in autophagy initiation, autophagosome-lysosome fusion, and lysosomal acidification.

Main Results:

  • Discovered that shibire (shi), encoding a dynamin protein, is essential for starvation-induced autophagy.
  • Demonstrated that shibire (shi) is required for lysosomal/autolysosomal acidification but not for autophagy initiation or fusion.
  • Identified roles for other endocytic components (clathrin, AP2) in autophagy and lysosomal function.

Conclusions:

  • Shibire (shi)/dynamin plays an indirect role in autophagy by regulating lysosomal acidification.
  • Endocytic machinery components influence autophagy and lysosomal function, expanding our understanding of their interplay.