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RNA-mediated genetic transformation in Aspergillus nidulans
Cellular and Molecular Biology
|January 1, 1989
Summary
Researchers achieved genetic correction in Aspergillus nidulans using RNA, demonstrating a novel RNA-dependent transformation. This "retrotransformation" restores wild-type genetic information in mutant cells.
Area of Science:
- Molecular Biology
- Genetics
- Mycology
Background:
- Genetic alterations in Aspergillus nidulans can be corrected.
- RNA molecules may play a role in genetic information transfer.
Purpose of the Study:
- To develop a model system for correcting genetic alterations in Aspergillus nidulans.
- To investigate the role of RNA in genetic transformation.
Main Methods:
- Protoplasts from a mutant Aspergillus nidulans strain were treated with total RNA from a wild-type strain.
- The effect of pancreatic ribonuclease on the transformation was assessed.
Main Results:
- A true genetic transformation occurred, correcting mutations (ribo, paba, bio, w, Acr).
- The transformation phenomenon was dependent on RNA, as it was abolished by ribonuclease treatment.
Conclusions:
- RNA can mediate the restoration of wild-type chromosomal information in mutant cells.
- The term "retrotransformation" is proposed for this RNA-mediated genetic correction process.