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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: Mar 24, 2026

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

Generating Mouse Models Using CRISPR-Cas9-Mediated Genome Editing.

Wenning Qin¹, Peter M Kutny¹, Richard S Maser¹

¹The Jackson Laboratory, Bar Harbor, Maine.

Current Protocols in Mouse Biology

|March 2, 2016

Summary

This summary is machine-generated.

CRISPR-Cas9 technology enables precise genome editing in mice by delivering key reagents into zygotes. This method facilitates the creation of mutant animals with targeted genetic modifications for research.

Keywords:

CRISPR genome editing knock-in knockout mouse model

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Published on: April 2, 2014

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

The CRISPR-Cas9 system, originating from bacterial and archaeal defense mechanisms, is a powerful tool for genome editing.
Its application has expanded to various model organisms, including mice, enabling precise genetic manipulation.

Purpose of the Study:

To outline the general considerations and experimental protocols for generating genetically modified mice using the CRISPR-Cas9 system.
To provide a guide for researchers interested in utilizing CRISPR-Cas9 for mouse genome engineering.

Main Methods:

Delivery of CRISPR-Cas9 reagents (guide RNA, Cas9 nuclease, and donor template) directly into mouse zygotes.
Utilizing guide RNA for target specificity and Cas9 nuclease to induce DNA double-strand breaks.
Employing donor oligonucleotides or plasmids with homologous sequences for precise mutation introduction.

Main Results:

Successful generation of mutant mice with targeted genetic modifications.
Demonstration of the CRISPR-Cas9 system's efficacy in mouse genome editing.
Establishment of protocols for creating genetically engineered mouse models.

Conclusions:

The CRISPR-Cas9 system offers a versatile and efficient method for creating genetically modified mice.
This technology significantly advances the study of gene function and disease modeling in mammals.
Detailed protocols are crucial for the successful implementation of CRISPR-Cas9 in mouse genetics research.