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[Cheek blood sampling of mice]
Shuaishuai Zhang1, Tianshou Li, Meirong Zang
1State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
Zhonghua Yi Xue Za Zhi
|March 3, 2016
Summary
This study demonstrates that cheek blood sampling in mice is a viable method for continuous blood testing. It allows for regular collection of sufficient blood volumes without negatively impacting the animals, making it an ethical and efficient technique.
Area of Science:
- Laboratory animal science
- Veterinary medicine
- Biomedical research
Background:
- Continuous blood testing is crucial for monitoring animal health and experimental outcomes.
- Traditional blood sampling methods can be invasive and stressful for laboratory animals.
Purpose of the Study:
- To evaluate the efficacy and animal welfare implications of cheek blood sampling in mice for serial blood collection.
- To establish cheek blood sampling as a reliable method for ongoing physiological monitoring in rodent models.
Main Methods:
- Cheek blood sampling was performed on 10 nine-week-old mice at 2-3 day intervals.
- Blood sample volumes were meticulously recorded.
- Animal behavior and reactions post-sampling were systematically observed.
Main Results:
- Each cheek blood sampling session yielded 0.3-0.6 ml of blood.
- Mice exhibited no adverse effects on their normal activities following blood collection.
- The sampling procedure was successfully repeated every 2-3 days without compromising animal welfare.
Conclusions:
- Cheek blood sampling in mice provides adequate blood volumes for analysis.
- The method is simple, easy to perform, and well-tolerated by the animals.
- This technique offers an ethical and practical approach to blood collection in laboratory mice, supporting continuous monitoring.
