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Analysis of peptide hormones of the hypothalamic pituitary adrenal axis using 'two-site' immunoradiometric assays
P J Lowry1, E A Linton, S C Hodgkinson
1Department of Biochemistry, University of Reading, UK.
Hormone Research
|January 1, 1989
Abstract:
The 'two-site' immunoradiometric assay has been used in a variety of situations to measure and characterize peptides of the hypothalamic pituitary adrenal axis. It has been found that this assay is more sensitive, specific and robust than classical radioimmunoassays and can provide additional evidence of the molecular forms of peptides without resorting to chromatography. The technique can also be used to measure peptides in plasma when a binding protein is present.