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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Updated: Mar 24, 2026

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
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Recent Progress in Molecular Recognition Imaging Using Atomic Force Microscopy.

Subhadip Senapati1, Stuart Lindsay1

  • 1Biodesign Institute, ‡Department of Chemistry and Biochemistry, and §Department of Physics, Arizona State University , Tempe, Arizona 85287, United States.

Accounts of Chemical Research
|March 3, 2016
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy (AFM) enables imaging and force measurements of single molecules under physiological conditions. Recent advancements in tip functionalization allow for simultaneous detection of multiple proteins, advancing bionanotechnology and diagnostics.

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Area of Science:

  • Bionanotechnology
  • Molecular Biophysics
  • Surface Science

Background:

  • Atomic force microscopy (AFM) offers high-resolution imaging and piconewton force measurements of single molecules.
  • AFM operates in aqueous media, allowing studies under physiological conditions without sample modification.
  • Molecular recognition imaging, a key AFM feature, maps specific molecular interactions via simultaneous topography and recognition (TREC) images.

Purpose of the Study:

  • To review the fundamentals of AFM recognition imaging.
  • To discuss AFM tip functionalization methods and recent advancements.
  • To explore future directions and diagnostic potential of AFM recognition imaging.

Main Methods:

  • Utilizing AFM for high-resolution imaging and force measurements.
  • Employing molecular recognition imaging with functionalized AFM tips.
  • Developing and applying novel heterofunctional triarm linkers for simultaneous multi-analyte detection.

Main Results:

  • Recognition imaging successfully applied to various systems like protein-protein and antigen-antibody interactions.
  • Demonstrated simultaneous detection of two different proteins using a novel triarm linker and "two-color" recognition imaging.
  • Established AFM's capability for detecting specific proteins in mixtures and monitoring biological phenomena in native states.

Conclusions:

  • AFM recognition imaging is a powerful tool for studying molecular interactions in bionanotechnology.
  • Novel tip functionalization strategies, such as heterofunctional triarm linkers, enhance AFM capabilities for complex biological systems.
  • AFM recognition imaging holds significant potential for advancing diagnostics and understanding multimolecular biological phenomena.