Related Experiment Video

Updated: Mar 24, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.5K

Flavone inhibits migration through DLC1/RhoA pathway by decreasing ROS generation in breast cancer cells

Wenzhen Zhu1, Long Ma1, Bingwu Yang1

  • 1Shandong Provincial Key Laboratory of Animal Resistant Biology, School of Life Sciences, Shandong Normal University, Jinan, 250014, China.

In Vitro Cellular & Developmental Biology. Animal
|March 4, 2016
PubMed

Insights

Flavone inhibits breast cancer cell migration by regulating the DLC1/RhoA pathway and decreasing reactive oxygen species (ROS). This compound also enhances DLC1 protein stability, offering a potential therapeutic strategy.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Tumor suppressor protein deleted in liver cancer 1 (DLC1) is a RhoGAP that inhibits cancer cell migration.
  • Reactive oxygen species (ROS) are known to regulate RhoGAP activity.

Purpose of the Study:

  • To investigate flavone's effect on ROS generation and the DLC1/RhoA pathway in breast cancer cells.
  • To determine if ROS mediates flavone-induced DLC1 upregulation.

Main Methods:

  • Utilized MCF-7 and MDA-MB-231 breast cancer cell lines.
  • Assessed ROS levels, DLC1 expression, RhoA activity, and cell migration.
  • Investigated the role of ROS by restoring levels with hydrogen peroxide.
  • Examined DLC1 protein stability and degradation.

Main Results:

  • Flavone decreased ROS production and inhibited cell migration via the DLC1/RhoA pathway.
  • Restoring ROS levels with hydrogen peroxide counteracted flavone's effects on DLC1, RhoA, and cell migration.
  • Flavone enhanced DLC1 stability by reducing its protein degradation.

Conclusions:

  • Flavone inhibits breast cancer cell migration by decreasing ROS and modulating the DLC1/RhoA pathway.
  • Flavone's mechanism involves enhancing DLC1 stability and reducing protein degradation.
  • These findings highlight flavone as a potential therapeutic agent targeting cancer cell migration.
Keywords:
Breast cancer cellsDLC1/RhoA pathwayFlavoneMigrationROS

More Related Videos

A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects TEdeff in Cancers10:49

A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects TEdeff in Cancers

Published on: September 26, 2019

6.1K
RhoC GTPase Activation Assay09:58

RhoC GTPase Activation Assay

Published on: August 22, 2010

13.1K

Related Concept Videos

Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.5K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
4.0K
Cancer Prevention02:59

Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
8.7K
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
7.3K
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
19.1K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.7K