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Updated: Mar 24, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Published on: April 4, 2018
The impact of structural genomics: the first quindecennial
Marek Grabowski1, Ewa Niedzialkowska1,2, Matthew D Zimmerman1
1Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, 1340 Jefferson Park Avenue, Jordan Hall, Room 4223, Charlottesville, VA, 22908, USA.
Structural genomics (SG) initiatives worldwide determined over 13,500 protein structures using high-throughput methods. These efforts enhanced data quality, accessibility, and reproducibility, benefiting biological and biomedical research.
Area of Science:
- Structural Biology
- Genomics
- Biophysics
Background:
- The early 2000s saw the rise of high-throughput technologies for protein structure determination.
- Significant global funding supported structural genomics (SG) consortia in establishing comprehensive pipelines.
Purpose of the Study:
- To detail the achievements of structural genomics programs between 2000-2015.
- To highlight the impact of these programs on protein structure coverage and scientific output.
Main Methods:
- Development of automated pipelines for target selection, protein production, crystallization, and structure determination (X-ray crystallography, NMR).
- Large-scale data generation and management using advanced software and databases.
Main Results:
- Over 13,500 protein structures determined, increasing structural coverage of the proteome.
- Publication of over 4400 scientific articles, with significant citation impact.
- Development of improved protocols and freely accessible data, enhancing research reproducibility.
Conclusions:
- Structural genomics initiatives successfully expanded the protein structure database.
- These programs significantly advanced biological and biomedical research through data sharing and methodological improvements.
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