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Updated: Mar 24, 2026
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Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
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Use of the CryoPredict algorithm to predict live birth from cryopreserved embryos.
Anthony J Marren1,2,3, Ying Ying Tan1,2, Bradley S de Vries3,4
1Department of Reproductive Endocrinology and Infertility, Royal Prince Alfred Hospital for Women and Babies, Camperdown, New South Wales, Australia.
Summary
Blastocyst re-expansion after warming predicts live birth rates. This method, combined with other factors, offers a more reliable way to assess embryo viability following cryopreservation and improve IVF success.
Area of Science:
- Reproductive biology
- Embryology
- In vitro fertilization (IVF)
Background:
- Assessing blastocyst viability post-cryopreservation is crucial for IVF success.
- Current methods like cell survival percentage are often unreliable due to blastocyst complexity.
- Faster blastocyst re-expansion after warming has been linked to higher pregnancy rates.
Purpose of the Study:
- To investigate the correlation between blastocoele re-expansion (degree and speed) and live birth rates after cryopreservation and warming.
- To identify reliable predictors of successful embryo transfer outcomes.
Main Methods:
- Retrospective cohort study of 757 frozen embryo transfer cycles.
- Data collected included patient demographics, IVF cycle details, cryopreservation parameters, and pregnancy outcomes.
- Analysis focused on factors associated with live birth, including blastocyst re-expansion.
Main Results:
- Female and male age at embryo creation were inversely associated with live birth.
- IVF factors significantly linked to live birth included fertilization method, embryo type and grade at cryopreservation, cell survival, and degree of blastocoele re-expansion.
- A predictive model, CryoPredict, was developed to estimate individual embryo transfer success probability.
Conclusions:
- The degree and speed of blastocoele re-expansion are valuable indicators for predicting live birth.
- Blastocoele re-expansion, alongside other parameters, enhances the accuracy of predicting IVF success post-cryopreservation.
