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Updated: Mar 24, 2026

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Published on: May 1, 2019
The RNA Helicase eIF4A Is Required for Sapovirus Translation
Myra Hosmillo1,2, Trevor R Sweeney2, Yasmin Chaudhry2
1Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Buk-gu, Gwangju, Republic of Korea.
Abstract:
The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.
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