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Updated: Mar 24, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Published on: November 5, 2018
Analysis of small-angle X-ray scattering data in the presence of significant instrumental smearing
Johan Bergenholtz1, Jeanette Ulama2, Malin Zackrisson Oskolkova3
1Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Göteborg, Sweden; Division of Physical Chemistry, Center of Chemistry and Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
Abstract:
A laboratory-scale small-angle X-ray scattering instrument with pinhole collimation has been used to assess smearing effects due to instrumental resolution. A new, numerically efficient method to smear ideal model intensities is developed and presented. It allows for directly using measured profiles of isotropic but otherwise arbitrary beams in smearing calculations. Samples of low-polydispersity polymer spheres have been used to show that scattering data can in this way be quantitatively modeled even when there is substantial distortion due to instrumental resolution.
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