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Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
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Basal body structure in Trichonympha.

Paul Guichard1, Pierre Gönczy2

  • 1Department of Cell Biology, University of Geneva, Geneva, Switzerland.

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|March 4, 2016
PubMed
Summary
This summary is machine-generated.

Trichonympha, a termite symbiont, possesses unusually long basal bodies. Cryo-electron tomography revealed novel structural features and a cartwheel composed of SAS-6 proteins, potentially conserved in other centrioles.

Keywords:
CartwheelCryo-tomographyLong centrioleTrichonympha

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Area of Science:

  • Cell Biology
  • Microbiology
  • Structural Biology

Background:

  • Trichonympha are symbiotic flagellates found in termites and wood-feeding cockroaches.
  • They possess numerous flagella for locomotion within the host hindgut.
  • Previous electron microscopy revealed exceptionally long basal bodies in Trichonympha compared to typical centrioles.

Purpose of the Study:

  • To investigate the 3D architecture of Trichonympha basal bodies in their native state.
  • To identify novel structural features of these elongated basal bodies.
  • To explore potential conservation of these features in other centrioles.

Main Methods:

  • Cryo-electron tomography was used to achieve high-resolution imaging (~40 Å) of Trichonympha basal bodies.
  • Analysis focused on the proximal region of the basal body.
  • Structural components were identified and characterized.

Main Results:

  • A detailed 3D map of the basal body proximal region was generated.
  • Novel features were identified, including proximal-distal polarity of the pinhead structure and the A-C microtubule linker.
  • The cartwheel structure was resolved as stacked rings, likely composed of 18 SAS-6 protein molecules each.

Conclusions:

  • Trichonympha basal bodies exhibit unique structural organization.
  • Identified features may represent conserved elements in centriole biology.
  • The study provides insights into the molecular composition and assembly of the cartwheel structure.