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Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells
Takeomi Mizutani1, Hisashi Haga1, Kazushige Kawabata1
1Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
Data in Brief
|March 4, 2016
Summary
This study investigated how modifying the adeno-associated virus integration site 1 (AAVS1) affects human fibroblast cell migration and cytoskeletal protein dynamics. The findings reveal impacts on cell movement and protein turnover.
Area of Science:
- Cell Biology
- Genetics
- Biophysics
Background:
- The adeno-associated virus integration site 1 (AAVS1) locus is a common site for gene insertion in research.
- Previous studies indicated AAVS1 modification influences cellular contractile force.
Purpose of the Study:
- To evaluate the effect of AAVS1 locus modification on human fibroblast cell migration speed.
- To assess the impact of AAVS1 modification on cytoskeletal protein mobility.
Main Methods:
- Comparison of wild-type human fibroblasts with fibroblasts engineered with a green fluorescent protein (GFP) gene at the AAVS1 locus.
- Analysis of cell nuclei movement to quantify migration speed.
- Fluorescence recovery after photobleaching (FRAP) to measure cytoskeletal protein turnover (myosin regulatory light chain).
Main Results:
- Cellular migration speed was compared between wild-type and modified fibroblasts.
- The turnover rate of fluorescently tagged myosin regulatory light chain was analyzed using FRAP.
- Data quantifies cellular dynamics in response to AAVS1 locus modification.
Conclusions:
- The study provides data on cell migration and cytoskeletal protein dynamics.
- Findings relate to the influence of AAVS1 locus transgene integration on cellular mechanics.
