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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
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Peptide [4]Catenane by Folding and Assembly.

Tomohisa Sawada1, Motoya Yamagami2, Kazuaki Ohara3

  • 1Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. tsawada@appchem.t.u-tokyo.ac.jp.

Angewandte Chemie (International Ed. in English)
|March 4, 2016
PubMed
Summary
This summary is machine-generated.

Researchers created a complex peptide [4]catenane using a novel folding and self-assembly strategy. This method simultaneously folds peptide fragments and directs their assembly into intricate molecular architectures.

Keywords:
macrocyclespeptidesself-assemblysilverstructure elucidation

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Area of Science:

  • Supramolecular Chemistry
  • Chemical Synthesis
  • Peptide Chemistry

Background:

  • Designing complex molecular architectures with precise topologies is a significant challenge in chemistry.
  • Peptide self-assembly offers a versatile platform for constructing sophisticated supramolecular structures.
  • Controlling the folding and assembly of peptide fragments is crucial for achieving desired topological complexity.

Purpose of the Study:

  • To synthesize a topologically complex peptide [4]catenane with a high crossing number.
  • To develop a simultaneous folding and metal-directed self-assembly strategy for peptide synthesis.
  • To investigate the formation of macrocyclic structures and their subsequent interlinking.

Main Methods:

  • Utilizing a folding and assembly strategy involving simultaneous folding and metal-directed self-assembly of peptide fragments.
  • Employing pyridines at the C- and N-termini of peptide fragments for metal ion coordination (Ag(I) or Au(I)).
  • Characterizing the resulting structures using crystallographic studies.

Main Results:

  • Successful synthesis of a peptide [4]catenane with a crossing number of 12.
  • Observation of a latent Ω-looped conformation in the Pro-Gly-Pro sequence upon metal ion binding.
  • Formation of four M3L3 macrocycles that interlocked to yield the complex [4]catenane topology.

Conclusions:

  • The developed strategy enables the construction of unprecedented peptide topologies.
  • Metal-directed self-assembly is effective in controlling peptide folding and macrocycle formation.
  • This work expands the possibilities for designing complex molecular architectures using peptides.