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Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
Published on: May 10, 2015
Function of Deubiquitinating Enzyme USP14 as Oncogene in Different Types of Cancer
Ying Zhu1, Chaoxiong Zhang, Changxin Gu
1Department of Respiratory Medicine, Changhai Hospital, Second Military Medical University, Shanghai, China.
Background/Aims:
Non-small cell lung cancer (NSCLC) tissues overexpress USP14, which promotes tumor cell proliferation and is associated with shorter overall survival time.
Methods:
The expression of USP14 was assayed in many types of cancers. USP14 was up-and down-regulated using appropriate plasmid or lentiviral vector constructs and its effects on proliferation, cell colony number, and apoptosis rate were measured. A human NSCLC cell line was inoculated into nude mice and the survival rates were recorded.
Results:
We found USP14 amplification and overexpression in many different cancers. The overexpression of USP14 in USP14 low-expression cell lines promoted cell proliferation and migration, whereas USP14 downregulation suppressed tumor cell proliferation, decreased tumor cell colony number, increased apoptosis rate, and decreased cell migration and invasion.
Conclusion:
USP14 plays an oncogenic role in various types of cancer, and may thus represent a new cancer therapy target.
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