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Related Experiment Videos

The Yersinia yop regulon.

G R Cornelis1, T Biot, C Lambert de Rouvroit

  • 1Unité de Microbiologie, Université Catholique de Louvain, Brussels, Belgium.

Molecular Microbiology
|October 1, 1989
PubMed
Summary
This summary is machine-generated.

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Yersiniae growth restriction at 37°C without calcium causes massive Yops protein release, regulated by the pYV plasmid and VirF. The exact role of calcium in Yops release is still unknown.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Yersiniae exhibit temperature-dependent growth regulation, specifically restricted growth at 37°C in low-calcium environments.
  • This growth restriction is linked to the secretion of effector proteins known as Yops (Yersinia outer proteins).
  • The genes encoding Yops and their regulatory elements are located on a large plasmid, pYV.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying Yersiniae growth restriction and Yops production.
  • To understand the role of the pYV plasmid and the virF gene in this thermoactivated regulon.
  • To explore the influence of calcium ions on the release of Yops proteins.

Main Methods:

  • Analysis of Yersiniae growth under varying temperature and calcium conditions.

Related Experiment Videos

  • Identification and characterization of genes involved in Yops production and secretion.
  • Molecular studies on the transcriptional regulation by the virF gene and its associated regulon.

    • Main Results:

    • Yersiniae growth is significantly inhibited at 37°C in the absence of calcium ions.
    • This inhibition correlates with the large-scale release of Yops proteins.
    • The pYV plasmid harbors the yop genes, forming a thermoactivated regulon controlled by virF.
    • VirF, a transcription activator, belongs to a family of regulators including those for arabinose and rhamnose operons.

    Conclusions:

    • The pYV plasmid and the virF gene are crucial for Yersiniae growth restriction and Yops production at 37°C.
    • Calcium ions play a significant, yet not fully understood, role in modulating Yops release.
    • VirF represents a key regulatory element in a conserved family of bacterial transcriptional activators.