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Microtubule structure at low resolution by x-ray diffraction.

E Mandelkow, J Thomas, C Cohen

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1977
    PubMed
    Summary
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    Cytoplasmic microtubules exhibit a wall structure approximately 70-150 Å thick. Analysis reveals distinct protofilament arrangements on the inner and outer surfaces of these essential cellular components.

    Area of Science:

    • Cell Biology
    • Structural Biology
    • Biophysics

    Background:

    • Cytoplasmic microtubules are crucial cytoskeletal polymers involved in intracellular transport and cell division.
    • Understanding their structural organization is key to elucidating their diverse functions.

    Purpose of the Study:

    • To analyze the detailed radial structure of hydrated cytoplasmic microtubules.
    • To characterize the surface features of the microtubule wall, including protofilament arrangement.

    Main Methods:

    • X-ray diffraction analysis of oriented hydrated cytoplasmic microtubules.

    Main Results:

    • The microtubule wall has a radial thickness of approximately 70 to 150 Å.
    • The outer surface shows vertical grooves separating 13 protofilaments and a 10-fold family of grooves.

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  • The inner surface is characterized by 10-start grooves, with protofilament subdivisions being less distinct.

    • Conclusions:

    • The microtubule wall possesses a complex, asymmetric structure with distinct outer and inner surface organizations.
    • These structural features likely influence microtubule interactions and dynamics within the cell.