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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Single-Cell Sequencing for Precise Cancer Research: Progress and Prospects.

Xiaoyan Zhang¹, Sadie L Marjani², Zhaoyang Hu¹

¹Hangzhou Cancer Institution, Hangzhou Cancer Hospital, Hangzhou, Zhejiang Province, P. R. China.

Cancer Research

|March 5, 2016

Summary

Single-cell sequencing reveals cancer cell heterogeneity and evolution, aiding in early detection and personalized therapy development. These advanced genomic techniques offer new insights into tumor biology and potential biomarkers.

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Area of Science:

Genomics
Cancer Biology
Molecular Oncology

Background:

Genomic technologies now allow detailed analysis of individual cancer cells.
Single-cell sequencing methods provide comprehensive insights into cancer genomes, transcriptomes, and epigenomes.

Purpose of the Study:

To review current single cancer-cell sequencing methods.
To highlight their applications, challenges, and future prospects in cancer research and therapy.

Main Methods:

Single-cell isolation techniques.
Whole genome and transcriptome amplification.
Epigenome profiling and multi-dimensional sequencing.
Next-generation sequencing and data analysis.

Main Results:

Unveiled significant cancer cell population heterogeneity and stochastic changes.
Identified novel driver mutations and complex clonal evolution mechanisms.
Enabled novel identification of biomarkers for various tumors.

Conclusions:

Single-cell sequencing advances early cancer detection and monitoring of rare cells (e.g., circulating tumor cells).
These methods are crucial for developing personalized and precise cancer therapies.