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Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Expression homeostasis during DNA replication.

Yoav Voichek1, Raz Bar-Ziv1, Naama Barkai2

  • 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.

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Summary
This summary is machine-generated.

Messenger RNA synthesis rate is buffered during DNA replication in yeast. This expression homeostasis relies on histone H3 K56 acetylation, which reduces transcription efficiency from replicated DNA.

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Yeast Genetics

Background:

  • Genome replication increases DNA template availability for transcription.
  • Early-replicating genes face this increase before late-replicating genes, posing questions about expression regulation.

Purpose of the Study:

  • To investigate how messenger RNA (mRNA) synthesis is affected by DNA replication.
  • To identify the molecular mechanisms maintaining expression homeostasis during S phase.

Main Methods:

  • Utilized budding yeast as a model organism.
  • Investigated gene expression levels during S phase.
  • Examined the role of histone H3 K56 acetylation and associated factors (Rtt109/Asf1).

Main Results:

  • Messenger RNA synthesis rate is buffered against gene dosage changes during S phase.
  • This expression homeostasis is dependent on histone H3 K56 acetylation by Rtt109/Asf1.
  • Deletion of these factors or mutation/deacetylation of H3K56 leads to increased gene expression proportional to replication timing.

Conclusions:

  • Histone H3 K56 acetylation on newly deposited histones suppresses transcription efficiency from replicated DNA.
  • This acetylation mechanism contributes to maintaining expression homeostasis during DNA replication.
  • The study provides molecular insights into genome stability and expression regulation.