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Sensitivity of Meloidogyne incognita and Rotylenchulus reniformis to Fluopyram
1University of Arkansas, Division of Agriculture, Cooperative Extension Service, Lonoke Research and Extension Center, Lonoke, AR 72086.
Abstract:
Fluopyram is a succinate dehydrogenase inhibitor (SDHI) fungicide that is being evaluated as a seed treatment and in-furrow spray at planting on row crops for management of fungal diseases and its effect on plant-parasitic nematodes. Currently, there are no data on nematode toxicity, nematode recovery, or effects on nematode infection for Meloidogyne incognita or Rotylenchulus reniformis after exposure to low concentrations of fluopyram. Nematode toxicity and recovery experiments were conducted in aqueous solutions of fluopyram, while root infection assays were conducted on tomato. Nematode paralysis was observed after 2 hr of exposure at 1.0 µg/ml fluopyram for both nematode species. Using an assay of nematode motility, 2-hr EC50 values of 5.18 and 12.99 µg/ml fluopyram were calculated for M. incognita and R. reniformis, respectively. Nematode recovery in motility was greater than 50% for M. incognita and R. reniformis 24 hr after nematodes were rinsed and removed from a 1-hr treatment of 5.18 and 12.99 µg/ml fluopyram, respectively. Nematode infection of tomato roots was reduced and inversely proportional to 1-hr treatments with water solutions of fluopyram at low concentrations, which ranged from 1.3 to 5.2 µg/ml for M. incognita and 3.3 to 13.0 µg/ml for R. reniformis. Though fluopyram is nematistatic, low concentrations of the fungicide were effective at reducing the ability of both nematode species to infect tomato roots.
Insights
Fluopyram, a succinate dehydrogenase inhibitor fungicide, showed nematistatic effects on plant-parasitic nematodes. Low concentrations reduced nematode infection of tomato roots, indicating potential for crop protection.
Area of Science:
- Agricultural Science
- Nematology
- Plant Pathology
Background:
- Fluopyram is an SDHI fungicide used for row crop disease management.
- Limited data exist on fluopyram's effects on Meloidogyne incognita and Rotylenchulus reniformis at low concentrations.
Purpose of the Study:
- To evaluate the toxicity, recovery, and infection effects of low fluopyram concentrations on M. incognita and R. reniformis.
- To determine fluopyram's efficacy in managing plant-parasitic nematodes in row crops.
Main Methods:
- Nematode toxicity and recovery assays were performed in aqueous fluopyram solutions.
- Root infection assays on tomato were conducted using fluopyram-treated nematodes.
- EC50 values for nematode motility were calculated.
Main Results:
- Nematode paralysis observed at 1.0 µg/ml fluopyram.
- 2-hr EC50 values were 5.18 µg/ml for M. incognita and 12.99 µg/ml for R. reniformis.
- Nematode infection of tomato roots was reduced at low fluopyram concentrations (1.3–13.0 µg/ml).
Conclusions:
- Fluopyram exhibits nematistatic properties.
- Low fluopyram concentrations effectively reduce nematode root infection in tomato.
- Fluopyram shows potential for managing plant-parasitic nematodes in row crops.
