Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

9.3K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
9.3K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

17.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.1K
DNA Topoisomerases02:02

DNA Topoisomerases

37.3K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
37.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellular transfection using rapid decrease in hydrostatic pressure.

Scientific reports·2024
Same author

Pathogenic CANVAS-causing but not nonpathogenic RFC1 DNA/RNA repeat motifs form quadruplex or triplex structures.

The Journal of biological chemistry·2023
Same author

Microfluidics-based PLGA nanoparticles of ratiometric multidrug: From encapsulation and release rates to cytotoxicity in human lens epithelial cells.

Heliyon·2023
Same author

Enhancement of the thermal stability of G-quadruplex structures by urea.

Biophysical chemistry·2023
Same author

Clinical Translation of Long-Acting Drug Delivery Systems for Posterior Capsule Opacification Prophylaxis.

Pharmaceutics·2023
Same author

Interaction of an anticancer benzopyrane derivative with DNA: Biophysical, biochemical, and molecular modeling studies.

Biochimica et biophysica acta. General subjects·2023

Related Experiment Video

Updated: Mar 24, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K

Concentration-dependent conformational changes in GQ-forming ODNs.

Yang Yun Li1, Rashid Abu-Ghazalah1, Bita Zamiri1

  • 1Department of Pharmaceutical Sciences, University of Toronto, Canada.

Biophysical Chemistry
|March 5, 2016
PubMed
Summary

Guanine-rich DNA (oligodeoxyribonucleotides) forming G-quadruplexes generally self-aggregate at high concentrations. Loop modifications did not prevent this concentration-dependent formation of parallel G-quadruplex aggregates.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.7K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.9K

Related Experiment Videos

Last Updated: Mar 24, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.4K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.7K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.9K

Area of Science:

  • Molecular Biology
  • Biophysics
  • Structural DNA

Background:

  • Guanine-rich oligodeoxyribonucleotides (ODNs) form G-quadruplexes, polymorphic four-stranded DNA structures stabilized by cations.
  • The human telomeric sequence ODN (H-Tel) forms various G-quadruplex topologies based on cation presence.
  • Previous studies showed H-Tel self-associates into higher-order structures at high concentrations.

Purpose of the Study:

  • To investigate if concentration-dependent self-aggregation of G-quadruplexes is a general phenomenon.
  • To explore the role of loop sequences in the self-association of G-quadruplex-forming ODNs.

Main Methods:

  • Circular dichroism (CD) spectroscopy.
  • Spectroscopically monitored heat denaturation.
  • Analysis of 19 modified H-Tel ODNs with altered loop sequences.

Main Results:

  • Concentration-dependent formation of parallel G-quadruplex aggregates was observed as a general phenomenon across modified ODNs.
  • The self-aggregation process appears to be independent of specific loop sequences.
  • Partially unfolded antiparallel structures may contribute to the observed self-association.

Conclusions:

  • The self-aggregation of G-quadruplexes is a general characteristic of guanine-rich ODNs.
  • Loop sequences are not the primary drivers of this concentration-dependent aggregation.
  • Further investigation into the role of partially unfolded structures in G-quadruplex aggregation is warranted.