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Isolation of Microsatellite Loci by Next-Generation Sequencing of the Critically Endangered Northern Bald Ibis,
Sarah Wirtz1, Christiane Böhm2, Johannes Fritz2
1From the Department of Biogeography, Trier University 54286 Trier, Germany (Wirtz and Hochkirch); Alpenzoo Innsbruck-Tirol, Weiherburggasse 37, 6020 Innsbruck, Austria (Böhm); Waldrappteam, LIFE+12-BIO_AT_000143, Schulgasse 28, 6162 Mutters, Austria (Fritz); and StarSEQ GmbH Mainz, J.-J.-Becherweg 30a, 55128 Mainz, Germany (Hankeln). s6sawirt@uni-trier.de.
Abstract:
The Northern Bald Ibis is one of the rarest bird species, extinct in Europe for 400 years and critically endangered worldwide. The European Union-co-financed LIFE+ project "Reason for Hope - Reintroduction of the Northern Bald Ibis in Europe" aims to reintroduce the species in Europe (Germany, Austria, Italy). In order to obtain information on the genetic diversity within zoo colonies and the reintroduced population, 15 polymorphic microsatellite markers, specific for the Northern Bald Ibis, Geronticus eremita (Linnaeus, 1785), have been isolated from next-generation sequencing (Illumina MiSeq) and are described here. The microsatellite primers were tested in 30 individuals and measures of genetic variability were calculated. Values for the observed heterozygosity ranged from 0.393 to 0.867, while expected heterozygosity ranged from 0.573 to 0.718. Ten out of 15 loci were in Hardy-Weinberg equilibrium and only one showed indication for the presence of null alleles. The newly developed PCR primers can be used to examine population genetic parameters, e.g. for future conservation genetic studies of this critically endangered bird species.
