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Related Experiment Video

Updated: Mar 24, 2026

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

Lysosomal cell death mechanisms in aging.

Raquel Gómez-Sintes¹, María Dolores Ledesma², Patricia Boya¹

¹Department of Cellular and Molecular Biology, Centro de Investigaciones Biologicas, CIB-CSIC, C/Ramiro de Maeztu 9, 28040 Madrid, Spain.

Ageing Research Reviews

|March 8, 2016

Summary

Aging impairs lysosome function, increasing cell sensitivity to damage and lysosomal cell death. This impacts tissue remodeling, immunity, and neurodegenerative diseases.

Keywords:

Aging Autophagy Cathepsin Lipids Lysosomal cell death Lysosomal membrane permeabilization Lysosomes

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Area of Science:

Cell Biology
Aging Research
Pathophysiology

Background:

Lysosomes are vital organelles for cellular homeostasis, regulating processes like nutrient response and autophagy.
Lysosomal cell death occurs when cathepsins are released into the cytoplasm due to lysosomal damage.
This cell death pathway is implicated in tissue remodeling, immune responses, and neurodegenerative conditions.

Purpose of the Study:

To review how aging affects lysosomal function.
To examine the link between aging, lysosomal damage, and cell death.
To discuss the role of these changes in physiological and pathological aging.

Main Methods:

Literature review focusing on aging and lysosomal function.
Analysis of studies on lysosomal membrane permeabilization and cathepsin release.
Synthesis of evidence on age-related alterations in lysosomal properties.

Main Results:

Aging alters the physical and chemical properties of lysosomes.
These alterations increase lysosomal sensitivity to stress and damage.
Lysosomal cell death mechanisms are more readily activated in aged cells.

Conclusions:

Aging significantly compromises lysosomal integrity and function.
Increased susceptibility to lysosomal cell death is a key feature of aging.
Understanding these age-related changes is crucial for addressing pathologies.