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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Published on: July 5, 2017
Use of cyclodextrins to recover catechin and epicatechin from red grape pomace
Santiago López-Miranda1, Ana Serrano-Martínez1, Pilar Hernández-Sánchez1
1Department of Food Technology & Nutrition, UCAM Universidad Católica de Murcia, Avenida de los Jerónimos s/n, 30107 Guadalupe, Murcia, Spain.
Abstract:
The capacity of cyclodextrins (CDs) to extract phenolic compounds from grape pomace was evaluated and compared with that of ethanol/water or aqueous extraction. The extraction method (stirring and ultrasound), temperature and time were also studied. Total phenolic compounds (TPC) and antioxidant activity were measured, and HPLC analysis was used to identify the phenolic compounds. The extracts obtained using the ethanol/water mixture presented the highest TPC content and antioxidant activity, followed by those obtained using CD solutions. The addition of CDs to the extractant agent had a selective effect on the extraction of catechin and epicatechin. The yield of catechin and epicatechin by using aqueous solutions of CDs was similar to that obtained using organic solvents as ethanol.
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