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Updated: Mar 24, 2026
A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
Data in support of proteomic analysis of pneumococcal pediatric clinical isolates to construct a protein array
Alfonso Olaya-Abril1, Ignacio Obando2, Manuel J Rodríguez-Ortega1
1Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain.
Insights
This study identified novel surface proteins of Streptococcus pneumoniae, a leading cause of child mortality. These proteins show potential as targets for new vaccines and affordable diagnostic tools for early infection detection.
Area of Science:
- Microbiology
- Proteomics
- Immunology
Background:
- Surface proteins are crucial for microbial interactions and are key targets for therapeutic and diagnostic development.
- Streptococcus pneumoniae is a significant pediatric pathogen causing over 1.5 million child deaths globally.
- Serodiagnostic tools offer affordable methods for early infection diagnosis.
Purpose of the Study:
- To identify potential surface protein targets from Streptococcus pneumoniae for vaccine and diagnostic development.
- To explore the serological reactivity of identified surface proteins using patient sera.
Main Methods:
- Proteomic analysis of pediatric clinical isolates of Streptococcus pneumoniae using trypsin shaving and LC/MS/MS.
- Production of 95 selected proteins as recombinant polypeptides for array printing.
- Probing of the protein array with patient sera to identify serodiagnostic antigens.
Main Results:
- Identification of 95 candidate surface proteins from Streptococcus pneumoniae.
- Demonstration of serological reactivity for several identified proteins using patient sera.
- Data deposited in the ProteomeXchange Consortium (PXD001740).
Conclusions:
- Surface proteins of Streptococcus pneumoniae are promising candidates for vaccine and diagnostic development.
- The identified antigens can be utilized for creating affordable serodiagnostic tools for early infection detection.
- This research contributes to the development of novel strategies against a major pediatric pathogen.
Abstract:
Surface proteins play key roles in the interaction between cells and their environment, and in pathogenic microorganisms they are the best targets for drug or vaccine discovery and/or development. In addition, surface proteins can be the basis for serodiagnostic tools aiming at developing more affordable techniques for early diagnosis of infection in patients. We carried out a proteomic analysis of a collection of pediatric clinical isolates of Streptococcus pneumoniae, an important human pathogen responsible for more than 1.5 million child deaths worldwide. For that, cultured live bacterial cells were "shaved" with trypsin, and the recovered peptides were analyzed by LC/MS/MS. We selected 95 proteins to be produced as recombinant polypeptides, and printed them on an array. We probed the protein array with a collection of patient sera to define serodiagnostic antigens. The mass spectrometry proteomics data correspond to those published in [1] and have been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository [3] with the dataset identifier PXD001740. The protein array raw data are provided as supplemental material in this article.
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