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An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
Published on: December 16, 2019
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Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method
Wassim Shebaby1, Eddie K Abdalla2, Fady Saad3
1Department of Natural Sciences, School of Arts & Sciences, Lebanese American University, Byblos, Lebanon, P.O. Box 36, Lebanon.
Data in Brief
|March 8, 2016
Summary
This study details a novel method for isolating mesenchymal cells from human fat without collagenase. The protocol yields cells that become more uniform in shape with extended culturing, crucial for stem cell research.
Area of Science:
- Biotechnology
- Cell Biology
- Tissue Engineering
Background:
- Mesenchymal cells are vital for regenerative medicine.
- Current isolation protocols often rely on enzymatic digestion, such as collagenase.
- Developing alternative, non-enzymatic methods is essential for broader applications.
Purpose of the Study:
- To present a detailed protocol for isolating mesenchymal cells from human adipose tissue without using collagenase.
- To characterize the morphology and growth patterns of these isolated cells.
- To provide data on cell isolation efficiency and doubling time.
Main Methods:
- Human adipose tissue was processed into small pieces (1-3 mm) after surgical cleaning.
- Fat explants were incubated in complete culture media for 5-7 days.
- Cell morphology, isolation efficiency, and doubling time were monitored during culture and subculturing.
Main Results:
- Mesenchymal cells successfully migrated from fat explants and exhibited exponential growth.
- Primary cells showed heterogeneous morphology, becoming more homogenous and fibroblastic with prolonged culture or subculturing.
- Data on cell isolation efficiency and doubling time were quantified.
Conclusions:
- A collagenase-free protocol effectively isolates mesenchymal cells from human fat.
- Cell culture duration and subculturing influence the morphological homogeneity of isolated mesenchymal cells.
- This method offers an alternative for obtaining adipose-derived mesenchymal stem cells (ASCs).
