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Updated: Mar 24, 2026
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Characterization of Methicillin Resistant Staphylococcus aureus isolated from human and animal samples in Egypt
M M Bendary1, S M Solyman1, M M Azab1
1Suez Canal University Department of Microbiology and Immunology, Faculty of Pharmacy Ismailia Egypt.
Abstract:
Staphylococcus aureus (S. aureus) has been one of the most problematic pathogens. Methicillin Resistant S. aureus (MRSA) has emerged as a major concern for both human and animal. Antibiotic resistance genes dissemination might be possible between human and animal bacteria. The aim of this study is to show phenotypic and genotypic diversity of human and animal MRSA isolates. Antibiogram typing and biofilm production were used as a primary phenotypic typing tool for the characterization of (40) animal and (38) human MRSA isolates. Genetic typing based on sequencing of 16S rRNA gene and virulence gene profiles were done. Antimicrobial resistance profiles of the animal isolates showed little evidence of widespread of resistance, although this was seen in many human isolates. The biofilm production was detected in higher percentage among animal isolates. Based on the genetic typing and multiple antibiotic resistance (MAR) index, the majority of animal isolates clustered into lineages that were not found in human isolates. Animal and human MRSA isolates showed diversity in antibiotic resistance and virulence gene profiles may be due to host adaptation or chances for contamination between the two hosts were not present in our study.
Insights
Human and animal Methicillin-Resistant Staphylococcus aureus (MRSA) isolates show distinct genetic and phenotypic differences. Animal MRSA exhibited higher biofilm production and less widespread antibiotic resistance compared to human strains.
Area of Science:
- Microbiology
- Infectious Diseases
- Genetics
Background:
- Staphylococcus aureus (S. aureus) is a significant pathogen, with Methicillin-Resistant Staphylococcus aureus (MRSA) posing a major threat to both human and animal health.
- The potential for antibiotic resistance gene dissemination between human and animal MRSA necessitates understanding their diversity.
Purpose of the Study:
- To investigate the phenotypic and genotypic diversity of human and animal MRSA isolates.
- To compare antimicrobial resistance profiles, biofilm production, and genetic relatedness between human and animal MRSA.
Main Methods:
- Phenotypic characterization using antibiogram typing and biofilm production assays.
- Genotypic characterization through 16S rRNA gene sequencing and virulence gene profiling.
- Calculation of the Multiple Antibiotic Resistance (MAR) index.
Main Results:
- Animal MRSA isolates showed less widespread antimicrobial resistance compared to human isolates.
- A higher percentage of animal MRSA isolates demonstrated biofilm production.
- Genetic typing revealed distinct clustering of animal MRSA lineages separate from human isolates.
Conclusions:
- Human and animal MRSA isolates exhibit significant diversity in antibiotic resistance and virulence gene profiles.
- Host adaptation or limited cross-contamination may contribute to the observed differences between animal and human MRSA populations.
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