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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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A conformation-induced fluorescence method for microRNA detection.

Sherry S Aw¹, Melissa Xm Tang², Yin Nah Teo³

¹Institute of Molecular and Cell Biology, 61 Biopolis Drive, 138673, Singapore syaw@imcb.a-star.edu.sg.

Nucleic Acids Research

|March 9, 2016

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This summary is machine-generated.

Researchers developed Pandan, a novel RNA sensor for detecting microRNAs. This innovation enables sensitive and specific quantification of microRNAs, aiding biomarker discovery and diagnostics.

Area of Science:

Molecular Biology
Biochemistry
RNA Biology

Background:

MicroRNAs regulate gene expression and are potential clinical biomarkers.
The small size of microRNAs poses challenges for direct detection and quantification.
Advancements in sensing techniques are crucial for understanding microRNA biology and developing diagnostics.

Purpose of the Study:

To engineer a novel RNA-based sensor for sensitive and specific microRNA detection.
To create a tool for quantifying microRNAs in complex biological samples.

Main Methods:

Engineered a new RNA construct, Pandan, based on the fluorescent RNA Spinach.
Modified Spinach by adding stem loops and altering its ends to create a microRNA-responsive sensor.
Designed Pandan with sequence-variable stem loops complementary to target microRNAs.

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Last Updated: Mar 24, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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Published on: October 7, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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Published on: April 14, 2015

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Main Results:

Pandan functions as a microRNA sensor, requiring target binding to reconstitute a fluorescent scaffold.
MicroRNA binding induced significant fluorescence changes, with a median fold change of approximately 50-fold.
Pandan RNA sensors demonstrated good signal-to-noise ratios and detected targets within complex RNA mixtures.

Conclusions:

Pandan is a versatile and sensitive RNA sensor for microRNA detection.
This technology facilitates microRNA research and holds potential for diagnostic applications.
The Pandan sensor overcomes limitations in direct microRNA detection.