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Methods for evaluating the acrosomal status of mammalian sperm
1Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis 95616.
Biology of Reproduction
|October 1, 1989
Abstract:
A full understanding of the acrosome reaction is central to understanding sperm function. Acrosomal status can be determined on living, motile sperm in only a few mammalian species. For other species, many light microscopic methods have been developed, including colored stains for bright-field microscopy, and probes for fluorescence microscopy. We review the existing methods and the criteria that should be considered in the choice of an assay.