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Novel Sequence Discovery by Subtractive Genomics

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Published on: January 25, 2019

Speciation in Cloudless Sulphurs Gleaned from Complete Genomes.

Qian Cong¹, Jinhui Shen¹, Andrew D Warren²

¹Departments of Biophysics and Biochemistry, University of Texas Southwestern Medical Center.

Genome Biology and Evolution

|March 9, 2016

Summary

This summary is machine-generated.

Complete genome sequencing reveals significant genetic differences between Cloudless Sulphur (Phoebis sennae) subspecies, Phoebis sennae eubule and Phoebis sennae marcellina, despite subtle phenotypic variations. Gene flow and introgression suggest adaptive advantages for caterpillar feeding.

Keywords:

Phoebis sennae comparative genomics introgression lepidoptera phylogeny speciation

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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

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Published on: March 8, 2018

Area of Science:

Evolutionary biology
Genomics
Zoology

Background:

Zoologists traditionally used phenotypes to study animal evolution.
Genomic data, especially complete genomes, offers deeper insights into speciation and genotype-phenotype links.
The Cloudless Sulphur butterfly (Phoebis sennae) provides a model for studying evolutionary divergence.

Purpose of the Study:

To sequence and assemble the complete genome of Phoebis sennae.
To compare the genomes of two distinct subspecies, Phoebis sennae eubule and Phoebis sennae marcellina.
To investigate genetic divergence, introgression, and potential adaptive mechanisms between these subspecies.

Main Methods:

Whole-genome sequencing and assembly of a wild-caught Phoebis sennae specimen.
Comparative genomics of six specimens representing eastern (P. s. eubule) and southwestern (P. s. marcellina) populations.
Phylogenetic analysis using complete genomes and comparison with other butterfly families.

Main Results:

Complete genomes of P. s. eubule and P. s. marcellina show significant differences, exceeding those between other butterfly species.
Key genetic differences were observed in histone methylation regulators, chromatin-associated proteins, circadian clock, and early development genes.
Introgression was detected, primarily from P. s. marcellina to P. s. eubule, with introgressed genes enriched in transmembrane transporters, potentially aiding nutrient uptake.

Conclusions:

Complete genomes provide a high-resolution view of evolutionary divergence in Phoebis sennae subspecies.
Genetic differences are more pronounced at the whole-genome level than suggested by phenotype or mitochondrial DNA.
Introgression of transporter genes may confer a selective advantage for larval feeding, highlighting adaptive evolution.
Genomic data reinforces the phylogenetic separation of Pieridae from Papilionidae.