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Updated: Jul 31, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Molecular and biological characterization of highly infectious transcripts from full-length cDNA clones of broad bean
Inmaculada Ferriol1, Silvia Ambrós2, Dorivaldo M da Silva3
1Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain; Plant Pathology Department, University of California Davis, Davis, CA 95616, USA.
Abstract:
Broad bean wilt virus 1 (BBWV-1), genus Fabavirus, has a genome composed of two single-stranded positive-sense RNAs of ∼5.8 (RNA1) and 3.4kb (RNA2). Full-length cDNA clones of both genomic RNAs (pBenR1 and pBenR2) from BBWV-1 isolate Ben were constructed under the control of the T7 promoter. In vitro derived capped transcripts were infectious in Nicotiana benthamiana, Chenopodium quinoa and Vicia faba plants. The biological activity of viral transcripts was not affected by extra bases at the 5'-terminus introduced during in vitro transcription. Virions derived from the infectious cDNA clones displayed similar viral infectivity and accumulation, as well as symptom induction as the wild-type BBWV-1 isolate.
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