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Bcmimp1, a Botrytis cinerea Gene Transiently Expressed in planta, Encodes a Mitochondrial Protein
David Benito-Pescador1, Daniela Santander2, M Arranz3
1Instituto Hispano-Luso de Investigaciones Agrarias - Departamento de Microbiología y Genética, Universidad de Salamanca Salamanca, Spain.
Frontiers in Microbiology
|March 9, 2016
Summary
Botrytis cinerea gene Bcmimp1, located in mitochondria, shows transient expression during tomato infection. Mutants had increased reactive oxygen species but unaltered infection ability, indicating Bcmimp1 is not a pathogenicity factor.
Area of Science:
- Plant Pathology
- Mycology
- Molecular Biology
Background:
- Botrytis cinerea is a significant necrotrophic fungal pathogen infecting numerous plant species.
- Understanding fungal gene expression during host interaction is crucial for identifying pathogenicity factors.
Purpose of the Study:
- To characterize the physiological status of Botrytis cinerea during tomato infection.
- To identify genetic factors contributing to fungal host cell infection.
Main Methods:
- Differential gene expression analysis of B. cinerea during tomato interaction.
- Bioinformatic analysis and gene cloning of Bcmimp1.
- Generation and characterization of ΔBcmimp1 mutants.
- Co-localization studies using BCMIMP1-GFP fusion protein.
Main Results:
- The gene Bcmimp1 exhibited transient expression, peaking during tissue colonization and maceration.
- Bioinformatic and experimental data localized BCMIMP1 to the mitochondrial inner membrane.
- ΔBcmimp1 mutants showed no significant changes in saprophytic growth or infection ability.
- Mutants displayed elevated reactive oxygen species levels, suggesting mitochondrial dysfunction.
Conclusions:
- Bcmimp1 is a mitochondrially localized protein with transient expression during Botrytis cinerea infection.
- Despite enhanced in planta expression, Bcmimp1 is not essential for pathogenicity.
- Disturbed mitochondrial function and increased reactive oxygen species in mutants highlight Bcmimp1's role in fungal physiology.
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