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Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Published on: July 27, 2022
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Hydroxyapatite from fish scale for potential use as bone scaffold or regenerative material.
Weeraphat Pon-On1, Panan Suntornsaratoon2, Narattaphol Charoenphandhu3
1Department of Physics, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Summary
Fish scale-derived hydroxyapatite (FSHA) exhibits superior bioactivity and biocompatibility compared to synthesized HA. FSHA shows enhanced apatite formation and osteoblast cell proliferation, indicating potential for bone scaffolds.
Area of Science:
- Biomaterials Science
- Materials Science
- Biotechnology
Background:
- Hydroxyapatite (HA) is a key material in bone regeneration.
- Synthesized HA (sHA) is a common standard, but novel sources are sought.
- Fish scales (FS) offer a potential sustainable source for HA production.
Purpose of the Study:
- To compare the physico-chemical, bioactivity, and biological properties of fish scale-derived hydroxyapatite (FSHA) with synthesized HA (sHA).
- To evaluate FSHA's potential as a bone scaffold material.
Main Methods:
- Characterization of FSHA and sHA (nanocrystal structure, Ca/P ratio).
- Bioactivity assessment using simulated body fluid (SBF) incubation.
- Biocompatibility evaluation via osteoblast cell adhesion, proliferation (MTT assay), and activity (ALP analysis).
Main Results:
- FSHA consists of flat-plate nanocrystals (15-20 nm width, 100 nm length) with a Ca/P ratio of 2.01 (Ca-rich).
- FSHA demonstrated greater apatite formation in SBF and enhanced osteoblast adhesion and proliferation compared to sHA.
- Osteoblast cells exhibited better spreading on FSHA, indicating improved biocompatibility.
Conclusions:
- Fish scale-derived hydroxyapatite (FSHA) is biologically superior to chemically synthesized HA.
- FSHA shows significant potential for applications in bone tissue engineering and regenerative medicine as a bone scaffold.
