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Different mast cell mediators produced by different mast cell phenotypes.

M F Gurish1, K F Austen

  • 1Department of Rheumatology and Immunology, Brigham and Women's Hospital, Boston, MA 02115.

Ciba Foundation Symposium
|January 1, 1989
PubMed
Summary
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Leukotriene C4 uses a probenecid-sensitive export carrier that does not recognize leukotriene B4.

Proceedings of the National Academy of Sciences of the United States of America·1992
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Isolation, characterization, and transcription of the gene encoding mouse mast cell protease 7.

Proceedings of the National Academy of Sciences of the United States of America·1992
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Purification of human leukotriene C4 synthase.

Proceedings of the National Academy of Sciences of the United States of America·1992
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Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig.

Journal of immunology (Baltimore, Md. : 1950)·1992
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IL-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of Trichinella spiralis-infected mice.

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Transcriptional regulation of the mucosal mast cell-specific protease gene, MMCP-2, by interleukin 10 and interleukin 3.

The Journal of biological chemistry·1992

Mast cells release inflammatory mediators via exocytosis. Researchers are studying mast cell development and regulation using in vitro systems and transformed cells to characterize key protein mediators.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Mast cells release diverse inflammatory mediators stored in secretory granules.
  • Exocytosis releases proteoglycan-macromolecular complexes containing neutral proteases (endo- and exopeptidases).
  • Mast cell phenotype is influenced by the microenvironment, with different mast cell types expressing distinct proteases.

Purpose of the Study:

  • To understand the development and regulation of mast cell phenotype.
  • To isolate and characterize cDNAs of preformed protein mediators involved in mast cell activation.
  • To develop molecular probes for characterizing mast cell proteases and proteoglycans.

Main Methods:

  • Development of an in vitro mast cell differentiation system.
  • Co-culture of in vitro-differentiated mast cells with fibroblasts to induce phenotypic shifts.

Related Experiment Videos

  • Utilizing Kirsten virus-transformed mast cells exhibiting varied phenotypes.
  • Isolation and characterization of complementary DNAs (cDNAs) for mast cell mediators.

    • Main Results:

    • Established systems to study mast cell differentiation and phenotypic plasticity.
    • Identified and characterized cDNAs for secretory granule proteoglycan peptide core, serine proteases, and carboxypeptidase.
    • Developed molecular probes for key mast cell components, including a carboxypeptidase A and a 28,000 Mr serine protease.

    Conclusions:

    • The microenvironment significantly influences mast cell phenotype.
    • Molecular characterization of mast cell mediators is achievable through cDNA analysis.
    • These findings provide tools for further investigation into mast cell biology and inflammatory processes.