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Related Experiment Videos

Enzyme immunoassay for oxytocin.

T Yasuda1, Z Mohri, Y Murakami

  • 1Research & Development Div., Sumitomo Pharmaceuticals Co., Ltd., Osaka, Japan.

Endocrinologia Japonica
|October 1, 1989
PubMed
Summary

A new enzyme immunoassay for oxytocin offers sensitive and reliable plasma measurements. This method, using horseradish peroxidase, shows high correlation with radioimmunoassay, making it suitable for clinical research.

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Area of Science:

  • Biochemistry
  • Endocrinology
  • Analytical Chemistry

Background:

  • Oxytocin is a crucial hormone involved in social bonding and reproduction.
  • Accurate measurement of plasma oxytocin levels is essential for physiological and clinical studies.
  • Existing methods like radioimmunoassay may have limitations in terms of cost, safety, or accessibility.

Purpose of the Study:

  • To develop and validate a competitive, double antibody enzyme immunoassay for quantifying oxytocin in plasma.
  • To assess the sensitivity, precision, and specificity of the developed enzyme immunoassay.
  • To compare the results obtained from the enzyme immunoassay with those from radioimmunoassay.

Main Methods:

  • Developed a heterologous enzyme immunoassay system using horseradish peroxidase conjugated to oxytocin.

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  • Produced rabbit anti-oxytocin serum via immunization with an oxytocin-bovine serum albumin complex.
  • Utilized SEP-PAK C18 cartridges for plasma extraction and measured oxytocin concentrations using the developed assay.

    • Main Results:

    • The enzyme immunoassay demonstrated a sensitivity of 4 microIU/tube (10 microIU/ml plasma).
    • Intra-assay and inter-assay coefficients of variation ranged from 6.8-15.9% and 8.5-16.7%, respectively.
    • High recovery (99-117%) and minimal cross-reactivity with vasopressins were observed, with strong correlation (r=0.90) to radioimmunoassay results.

    Conclusions:

    • The developed enzyme immunoassay provides a sensitive, precise, and specific method for measuring plasma oxytocin.
    • This assay is a viable alternative to radioimmunoassay for oxytocin determination in biological samples.
    • The validated assay can be effectively used in research settings for studying oxytocin's role in various physiological conditions.