New Small Plasmid Harboring blaKPC-2 in Pseudomonas aeruginosa
Renata Galetti1, Leonardo Neves Andrade2, Michael Chandler3
1Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil regaletti@hotmail.com.
Antimicrobial Agents and Chemotherapy
|March 9, 2016
Abstract:
The aim of this study was to characterize the genetic context of blaKPC-2 in Pseudomonas aeruginosa sequence type 244 from Brazil. The blaKPC-2 gene was detected in a new small plasmid, pBH6. Complete sequencing revealed that pBH6 was 3,652 bp long and included the Tn3 resolvase and Tn3 inverted repeat (IR), a partial copy of ISKpn6, and a putative ori region but no rep genes. pBH6 replicated stably into Escherichia coli strain DH10B and P. aeruginosa strain PAO.


