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Updated: Mar 24, 2026
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
Published on: April 23, 2012
Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in
Molly E Church1, Florante N Dela Cruz1, Kevin Kim2
1UC Davis, School of Veterinary Medicine, Department of Pathology, Microbiology, and Immunology, USA.
Abstract:
Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
