Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative modulation of a spatial enhancer through the biophysical properties of a transcription factor binding site.

Science advances·2026
Same author

FETCH enables fluorescent labeling of membrane proteins in vivo with spatiotemporal control in <i>Drosophila</i>.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

A critical affinity window for IgSF proteins DIP-α and Dpr10 is required for proper motor neuron arborization.

Genes & development·2025
Same author

Predicting the DNA binding specificity of transcription factor mutants using family-level biophysically interpretable machine learning.

Nucleic acids research·2025
Same author

Decoding neuronal wiring by joint inference of cell identity and synaptic connectivity.

bioRxiv : the preprint server for biology·2025
Same author

Members of the DIP and Dpr adhesion protein families use cis inhibition to shape neural development in Drosophila.

PLoS biology·2025

Related Experiment Video

Updated: Mar 24, 2026

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster
08:19

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

Published on: December 19, 2011

12.3K

Streamlined scanning for enhancer elements in Drosophila melanogaster.

Roumen Voutev1, Richard S Mann1

  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY.

Biotechniques
|March 10, 2016
PubMed
Summary

Scientists developed a rapid method to find enhancer elements, which are DNA sequences regulating genes, by scanning large genomic regions using transgenic reporter genes. This technique aids in understanding gene regulation, especially for complex expression patterns.

Keywords:
CRMD. melanogasterenhancerreporter construct

More Related Videos

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

8.0K
Chromatin Immunoprecipitation ChIP using Drosophila tissue
13:47

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Published on: March 23, 2012

26.0K

Related Experiment Videos

Last Updated: Mar 24, 2026

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster
08:19

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

Published on: December 19, 2011

12.3K
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

8.0K
Chromatin Immunoprecipitation ChIP using Drosophila tissue
13:47

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Published on: March 23, 2012

26.0K

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Enhancer elements regulate gene transcription, often located far from the transcription start site.
  • Complex three-dimensional chromatin organization and insulators define enhancer activity range.
  • Identifying all cis-regulatory modules (CRMs) for genes with complex expression requires extensive genomic locus testing.

Purpose of the Study:

  • To develop a fast and efficient method for identifying enhancer elements.
  • To enable scanning of large genomic regions for regulatory elements.
  • To facilitate the study of genes with complex and dynamic expression patterns.

Main Methods:

  • Utilizing transgenic reporter genes to assay enhancer activity.
  • Scanning large genomic regions for potential enhancer elements.
  • Developing a high-throughput method for enhancer identification.

Main Results:

  • A new, efficient method for enhancer element identification was established.
  • The method allows for rapid scanning of extensive genomic areas.
  • Successfully identified enhancer elements regulating gene expression.

Conclusions:

  • The developed method provides a fast and efficient approach for enhancer discovery.
  • This technique is valuable for characterizing cis-regulatory modules, particularly for complex gene expression.
  • Facilitates a deeper understanding of gene regulation in eukaryotic organisms.